I previously introduced a class of antibiotic-free small DNA vectors termed “Petit Plasmids”. These vectors can be maintained and replicated within engineered amino acid auxotrophic bacterial strains cultivated in a medium lacking one or more essential amino acids. In large-scale fermentation systems, these growing cells can scavenge lysed cel…[Read more]

Safety switch systems represent gene expression units capable of transcription and translation, resulting in the production of polypeptides that induce cell death upon exposure to specific activating ligands. This discourse introduces RNAse-based safety switches, collectively known as DESNase (a combination of the words ‘destabilized’ and ‘RN…[Read more]

The utilization of antibiotic resistance genes as selectable markers is discouraged due to the risks of plasmids uptake by endogenous bacterial flora. This could lead to the emergence and dissemination of antibiotic-resistant microbes. Herein, I disclose novel antibiotic resistance gene-free DNA plasmids and provide comprehensive details about the…[Read more]

The global burden of cancer remains high, with tens of millions of new cases being diagnosed and millions of lives being lost to the disease each year. Chemotherapy and targeted therapies have limitations and can cause serious side effects. Nucleic acids, including DNA and RNA, offer immense potential as innovative therapeutics for the treatment…[Read more]