I report the results of a study that investigated the combined effects of gender and age on luminous efficiency as measured by heterochromatic flicker photometry. Luminous Efficiency functions were derived for 13 male and 14 female color normal subjects between the ages of 8 and 72 years. Three age groups were used for recruitment and analysis: under 18 years (young), 21-45 years (adult) and over 60 years (older adult). Stimuli were 2° circular patches of monochromatic light (420 – 676 nm) presented in Newtonian-view. Each stimulus alternated at 20 Hz and was adjusted by observers to match the brightness of a 3.4 cd/m2 broadband reference patch of equal size. Normalized relative luminous efficiency data were analyzed across wavelengths by multivariate measures analysis of variance with gender and age group as fixed factors. Univariate analyses were repeated for each age group with gender as a fixed factor and again for males and females with age group as a fixed factor. There were no main or interaction effects of gender or age group across wavelengths. Older adult subjects did have significantly lower relative efficiency at low wavelengths (420 and 450 nm). Significant correlations were also found between relative efficiency and age for half of the tested wavelengths. Correlation results were similar when analyzed separately for males and females. These results provide support for requiring age-matched samples in studies of luminous efficiency, particularly for very young subjects. There was, however, little evidence that males differed from females overall or for any age group.